Methods of genotyping of pathogenic microorganisms

Abstract

There are various phenotypic methods for typing microorganisms, the deficiencies of which have led to the
formation of new innovative typing methods on the basis of microorganisms‘ DNA sequence. These methods minimize problems
with discriminating power and reproducibility and, in some cases, allow the creation of large compatible databases of specified
organisms. In this review a description of some molecular genetic methods of typing are given including those based on the
polymerase chain reaction: MLVA, rep-PCR, WGS. This data can be used by researchers in their practical activities, as they
provide some information on the content and properties of genotypic methods.
The application of these methods enables to carry out long-term epidemiological surveillance of infectious diseases, which in
turn will lead to a significant reduction in the number of infected people and elimination of the ways of spreading diseases.