Modification of plant genomes using DNA technologies: progress in the delivery of exogenous DNA

Abstract

The article reviews data on the delivery of exogenous DNA fragments used in DNA technologies as expression cassettes for heterologous genes in the creation of transgenic plants, or as tools for modifying plant genomes using genome editing methods. The main focus is on in planta delivery methods that allow researchers to bypass the plant cultivation stage, which includes the disintegration of plant tissues into callus cultures followed by the in vitro regeneration of plant regenerants. It is this stage of plant genome modification using modern DNA technologies that is currently attracting the attention of many research groups. Data are presented on the prospects of using viral vectors for the transient delivery of genome editing tools into plant cells. The possibilities and some disadvantages of this technology are discussed, as well as the prospects for its application to agricultural plant species.