Testing the process of accumulation of the virus sheep pox


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Authors

  • K.N. Zhaslanova LLP “Biotron Group”
  • G.M. Salkhozhayeva L.N. Gumilyov Eurasian National University
  • Zh.A. Rakhimzhanova L.N. Gumilyov Eurasian National University
  • M.K. Tynykulov L.N. Gumilyov Eurasian National University
  • I.A. Puntus LLP “Biotron Group”
  • K.M. Urazov LLP “Biotron Group”

Keywords:

sheep pox virus, vaccine, strain, culture, titer, monolayer, incubation, infection

Abstract

The article presents the results of adaptation and development of optimal parameters of accumulation of sheep
pox virus strain on the transplanted cell cultures 3-KG, YADK-04 and PO-2. Infectious activity in case of infection with VSP
on a complete formed monolayer was 5.0-5.75 lg TCD 50 /cm 3 on the YADK line and 5.5-6.0 lg TCD 50 /cm 3 on 3-KG, with
a plurality of infection 0.1-0.5 TCD 50 /CL., incubation time was 72-96 hours. The method of determining the infectious titer
of the virus by test-tube and microplate methods is worked out, the correlation analysis is carried out. Analysis of the results
of determining the infectious activity of the smallpox virus showed comparability of the data with a discrepancy of + 0,1-0,5 lg
(+0,25) towards the microplate method (n=7), which indicates its higher sensitivity and accuracy.

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Published

2026-06-08

How to Cite

Zhaslanova К. ., Salkhozhayeva Г. ., Rakhimzhanova Ж. ., Tynykulov М. ., Puntus И. ., & Urazov К. . (2026). Testing the process of accumulation of the virus sheep pox. BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series, 126(1), 37–45. Retrieved from https://bulbio.enu.kz/index.php/main/article/view/1007

Issue

Vol. 126 No. 1 (2019)

Section

Статьи