Оптимальные технологические параметры культивирования линии клеток ВНК-21


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Authors

  • I.A. Puntus LLP “Biotron Group”
  • K.N. Zhaslanova LLP “Biotron Group”
  • G.M. Salkhozhayeva L.N. Gumilyov Eurasian National University
  • M.K. Tynykulov L.N. Gumilyov Eurasian National University
  • K.M. Urazov LLP “Biotron Group”

DOI:

https://doi.org/10.32523/2616-7034-2020-130-1-37-46

Keywords:

линии клеток ВНК-21 и ВНК-21(сl-13), стационарные, роллерные, роллерные монослойно-суспензионные методы, модификация, вакцина, культура, титр, монослой, инкубация

Abstract

The article presents the results of cultivation of cell lines VNK-21 and VNK-21(сl-13) stationary, roller, roller monolayer-suspension methods. Optimal technological parameters of cell cultivation are given and growth modified enriched nutrient media are selected for the tested cell lines. Aspects of scaling the process of cell culture are considered. TheoptimalincubationconditionsoftheVNK-21(сl-13)linewereselected: constanttemperature+37± 0.1 0 Cinthethermal room, roller rotation speed 0.5-0.8 rpm, modified enriched nutrient medium FGM-C+DMEM (3:1), sieving multiplicity 1:15-1:25 or sowing concentration 85-150 thousand CL/ml, cell removal method - versin+trypsin (9:1). A roller monolayer-suspension cultivation method is proposed for accumulation of biomass of VNK-21(сl-13)cells in rollers.

 

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Published

2025-06-20

How to Cite

Puntus И., Zhaslanova К., Salkhozhayeva Г., Tynykulov М., & Urazov К. (2025). Оптимальные технологические параметры культивирования линии клеток ВНК-21. BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series, 130(1), 37–46. https://doi.org/10.32523/2616-7034-2020-130-1-37-46

Issue

Vol. 130 No. 1 (2020)

Section

Статьи