Isolation and characterization of umbilical cord-derived mesenchymal stem cells

Abstract

Mesenchymal stem cells (MSCs) isolated from human umbilical cord have been studied by scientists for more than 10 years due to their regenerative potential. Identification of the phenotype of these cells is very important because it shows the presence of properties of stem cells. This study aims to identify the phenotype of MSCs that were obtained from the human umbilical cord. MSCs were isolated from human umbilical cord and cultured in complete α-МЕМ medium. The colony-forming capacities of the obtained MSCs were analyzed by colony-forming assay. The phenotype of the obtained MSCs was evaluated using flow cytometry. Isolated MSCs showed the typical morphology of MSCs and demonstrated the ability to form colonies in the CFU assay. Obtained MSCs showed the expression of surface markers of normal MSCs. Thus, the cells that were isolated from the human umbilical cord confirmed the presence of the phenotype of MSCs.