Cell culture in vitro in studying of intraspecies genetic polymorphism in Pinus sibirica Du Tour

Abstract

The authors have studied characteristics of callusogenesis in Siberian pine (Pinus sibirica) trees of two populations: the control population and the population of elite (plus) trees. For the population of plus trees, the appearance of callus culture on the explants of buds and needles occurred earlier than in the control population. Differences in the growth rate of callus cultures of the studied populations have been established for the first 2 months of cultivation. It is concluded that the in vitro cell culture method can serve as an effective method for differentiating of P. sibirica genotypes.