Search for promising protease producers used for molecular diagnostics

Abstract

The article considers that the majority of biotechnologies in industry and agriculture are based on catalytic processes carried out by enzymes of microbial origin. Special importance is currently attached to enzyme preparations of proteolytic action, which is due to the significant opportunities for their multi-purpose use in various industries. Particularly relevant in recent years is the use of proteolytic enzymes in basic and applied medical research-the detection of vital proteins in the human body. In this paper, we selected the most promising strain - a producer of proteolytic enzymes. The activity of proteolytic enzymes formed by local strains isolated from natural sources (soil of the Turkestan region) was studied. Active producers of proteases are bacteria, microscopic fungi and actinomycetes. Microscopic fungi of the genus Aspergillus have the greatest ability to biosynthesize proteases. As a seed material, an aqueous spore suspension obtained after the growth of fungi was used. The choice of the producer strain is determined by its ability to provide sufficiently high levels of protease activity in the fermentation medium, the rate of formation of enzymes per unit mass of the substrate used, as well as the cost of the substrates themselves.