Implementation of multiphoton intravital microscopy in mesenteric and coronary artery research

Abstract

Advancements in intravital imaging technologies, particularly multiphoton microscopy, have significantly improved our ability to visualize and understand the dynamic interactions within vascular structures and various cell types in real time. However, traditional in vitro techniques—such as isometric myography—remain standard tools for assessing vascular reactivity. This study aims to develop and integrate both in vivo and in vitro methodologies to evaluate the functional and structural states of smooth muscle and endothelial layers in mesenteric vessels at the cellular level. Using native microscopy techniques, we assess morphological changes and correlate them with pharmacological responses in both mesenteric and coronary arteries. A key objective is to compare the capabilities and limitations of isometric myography and advanced multiphoton microscopy in analyzing vascular contractility and relaxation responses. The combined use of these techniques is expected to increase data quality, reduce animal usage, and support longitudinal studies. This integrative approach also enables the evaluation of both acute and chronic effects of pharmacological agents under near-physiological conditions, offering a more comprehensive understanding of vascular function.