Study of quality and quantitative composition of Berberis iliensis and Berberis integerrima fruits extracts, preservation of the genetic material in cryobank


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Authors

  • N.V. Romadanova Institute of Plant Biology and Biotechnology
  • K.A. Eshbakova Institute of the Chemistry of Plant Substances named acad. S. Yu Yunusov
  • L.N. Karasholakova Institute of Plant Biology and Biotechnology
  • I.A. Machmutova Institute of Plant Biology and Biotechnology
  • K.T. Abidkulova al-Farabi Kazakh National University
  • S.V. Kushnarenko Institute of Plant Biology and Biotechnology

Keywords:

Berberis iliensis, Berberis integerrima, in vitro collection, seeds, shoot tips, cryopreservation, phytochemical analysis

Abstract

Two barberry species have been studied and preserved: Berberis iliensis M. Pop., Berberis integerrima Bunge.
As a result of work the in vitro collection consisting of 33 accessions of these species was established. Obtaining in vitro
collection was placed for medium-term storage at 4 ◦ C. Optimal duration of cold storage is 12 months. Cryobank of shoot
tips and seeds was created. Cryobank of seeds created at temperatures: 1)-196 ◦ C, seeds germination after defrosting– 80
100%; 2)-20 ◦ C, seed germination after defrosting the average is 86,7%. Positive effect of storage at low temperatures on
seeds germination was revealed. To study the qualitative and quantitative chemical composition 11 accessions of two barberry
species were investigated. Fatty acids, fatty acids esters, alkanes, alkenes, alkadienes, aromatic hydrocarbons, aldehydes, fatty
alcohols, triterpenoids, ketones, silicones, vitamins K, K 1 , K 2 were identified in studied accessions. In some barberry accessions
carotenoid squalene, hydrocarbons 28-Nor-17-alpha and amide pyridine-3-carboximidamide were detected.

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Published

2026-06-07

How to Cite

Romadanova Н. ., Eshbakova К. ., Karasholakova Л. ., Machmutova И. ., Abidkulova К. ., & Kushnarenko С. . (2026). Study of quality and quantitative composition of Berberis iliensis and Berberis integerrima fruits extracts, preservation of the genetic material in cryobank. BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series, 127(2), 22–36. Retrieved from https://bulbio.enu.kz/index.php/main/article/view/995

Issue

Vol. 127 No. 2 (2019)

Section

Статьи