Modification of Plant Genomes by Genetic Engineering and Genome Editing: Delivery of Exogenous DNA

Abstract

The article reviews data on the development and improvement of methods for delivering exogenous DNA fragments into the genomes of various plant species in the postgenomic era of biology. The features of exogenous DNA delivery, depending on the stage of plant cell cultivation in vitro are presented. The emphasis is placed on the development of methods that ensure the delivery of expression cassettes into plant cell genomes in planta, excluding the disintegration of plant tissues to cell cultures with subsequent restoration of transformant plants, i.e., bypassing the in vitro stage. Data are presented on the success of the floral dip method for both model plants Arabidopsis thaliana, and some other species that are representatives of six families of higher plants. Data are presented on the demand for the floral dip method in connection with the growing interest in plant modification by genome editing methods.