Study the level of thymus stromal lymphopoietin in the blood of persons exposed to asbestos

Abstract

Asbestos dust is classified as a carcinogen by the International Agency for Research on Cancer. Asbestos particles, binding cations, leach the cell environment. The initiation of the release of dust particles activates the NF-kB signaling cascade, which in turn includes the synthesis of pro-inflammatory cytokines, such as thymus stromal lymphopoietin (TSLP). The increased level of these cytokines is directly related to malignant processes in the body.

The aim of the study is to study the level of TSLP in the blood serum of people exposed to asbestos.

The material for the study was cytokines isolated from the blood serum of 40 exposed to asbestos and 50 healthy people. Peripheral blood samples were collected from workers of the Zhitikara asbestos mining company of the city of Kostanay. The content of the main TSLP levels in blood serum was determined by ELISA the Human TSLP ELISA Kit (Biorbyt Ltd, UK, No. orb138077). The optical density of the reaction products was determined using a spectrophotometer with a wavelength of 450 nm.

The concentration of TSLP in the blood serum were increased 3 times in persons exposed to asbestos compared to the control group (p = 0.000011).

The concentration of the level of TSLP can serve as a diagnostic biomarker of a number of diseases that are induced by asbestos.