In vitro micropropagation of Canadian serviceberry (Amelanchier canadensis): optimization of cytokinin-auxin combinations for shoot induction

Abstract

The article presents a study on optimizing the conditions for micropropagation of three varieties of Amelanchier canadensis in vitro. It was found that modification of the Murashige and Skoog nutrient medium by introducing 6-benzylaminopurine (BAP) in various concentrations has a differentiated effect on the morphogenetic activity of explants. The most effective combination for Martin and Slate varieties was the use of BAP at a concentration of 1.0 mg/l and GA₃ – 0.5 mg/l, while for the Northline variety the optimal concentration was 0.5 mg/l BAP and 0.5 mg/l GA₃. Increasing the BAP concentration above 1.0 mg/l led to a decrease in both the length and the number of microshoots. The results obtained can be applied to large-scale propagation and conservation of these varieties of serviceberry.